iκbα wt Search Results


iκbα wt  (Addgene inc)
91
Addgene inc iκbα wt
Iκbα Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/i%CE%BAb%CE%B1+wt/pDONR223_NFKBIA_WT+(Plasmid+%2381833)/pm35405016-446-0-6
Average 91 stars, based on 1 article reviews
iκbα wt - by Bioz Stars, 2026-11
91/100 stars
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pcmv-iκbα wild type (wt  (Becton Dickinson)
90
Becton Dickinson pcmv-iκbα wild type (wt
Shh produced by LPS-stimulated THP-1 cells promotes proliferation of pancreatic cancer cells A, THP-1 cells were cultured in the presence or absence of LPS for 24 h. THP-1 cells were then washed intensively to eliminate LPS and the cell number was readjusted. AsPC-1 or SUIT-2 was cocultured with non-stimulated THP-1 cells, LPS-stimulated THP-1 cells or LPS-stimulated THP-1 cells with 5E1 or IgG antibody for 4 days. Total cell number was counted with a Coulter counter. The fractions of THP-1 cells and PDAC cells were determined using a FACSCaliburTM and the absolute number of PDAC cells was calculated. b AsPC-1 or SUIT-2 was transfected with siRNA for Shh or control siRNA by Lipofectamine for 36 h, and the expression of Shh mRNA was evaluated by real-time PCR (left panels). Shh-silenced AsPC-1 or SUIT-2 was cocultured with non-stimulated THP-1 cells, LPS-stimulated THP-1 cells, or LPS-stimulated THP-1 cells with 5E1 or IgG antibody for 4 days. Total cell number was counted with a Coulter counter. The fractions of THP-1 cells and PDAC cells were determined using a FACSCaliburTM and the absolute number of PDAC cells was calculated. Bars SD.*P < 0.05. c AsPC-1 or SUIT-2 was transfected with siRNA for Smo or control siRNA by Lipofectamine for 36 h, and the expression of Smo mRNA was evaluated by real-time PCR (left panels). <t>Wild-type,</t> control siRNA or Smo-siRNA transfected AsPC-1 or SUIT-2 was cocultured with LPS-stimulated THP-1 cells for 4 days. Total cell number was counted with a Coulter counter. The fractions of THP-1 cells and PDAC cells were determined using a FACSCaliburTM and the absolute number of PDAC cells was calculated. Bars SD. *P < 0.05
Pcmv Iκbα Wild Type (Wt, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/i%CE%BAb%CE%B1+wt/pcmv+i%CE%BAb%CE%B1m/pmc11030800-116-1-11
Average 90 stars, based on 1 article reviews
pcmv-iκbα wild type (wt - by Bioz Stars, 2026-11
90/100 stars
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pgl3-iκbα-wt  (Promega)
90
Promega pgl3-iκbα-wt
Shh produced by LPS-stimulated THP-1 cells promotes proliferation of pancreatic cancer cells A, THP-1 cells were cultured in the presence or absence of LPS for 24 h. THP-1 cells were then washed intensively to eliminate LPS and the cell number was readjusted. AsPC-1 or SUIT-2 was cocultured with non-stimulated THP-1 cells, LPS-stimulated THP-1 cells or LPS-stimulated THP-1 cells with 5E1 or IgG antibody for 4 days. Total cell number was counted with a Coulter counter. The fractions of THP-1 cells and PDAC cells were determined using a FACSCaliburTM and the absolute number of PDAC cells was calculated. b AsPC-1 or SUIT-2 was transfected with siRNA for Shh or control siRNA by Lipofectamine for 36 h, and the expression of Shh mRNA was evaluated by real-time PCR (left panels). Shh-silenced AsPC-1 or SUIT-2 was cocultured with non-stimulated THP-1 cells, LPS-stimulated THP-1 cells, or LPS-stimulated THP-1 cells with 5E1 or IgG antibody for 4 days. Total cell number was counted with a Coulter counter. The fractions of THP-1 cells and PDAC cells were determined using a FACSCaliburTM and the absolute number of PDAC cells was calculated. Bars SD.*P < 0.05. c AsPC-1 or SUIT-2 was transfected with siRNA for Smo or control siRNA by Lipofectamine for 36 h, and the expression of Smo mRNA was evaluated by real-time PCR (left panels). <t>Wild-type,</t> control siRNA or Smo-siRNA transfected AsPC-1 or SUIT-2 was cocultured with LPS-stimulated THP-1 cells for 4 days. Total cell number was counted with a Coulter counter. The fractions of THP-1 cells and PDAC cells were determined using a FACSCaliburTM and the absolute number of PDAC cells was calculated. Bars SD. *P < 0.05
Pgl3 Iκbα Wt, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/i%CE%BAb%CE%B1+wt/pgl3+basic/pm21376060-60-18-27
Average 90 stars, based on 1 article reviews
pgl3-iκbα-wt - by Bioz Stars, 2026-11
90/100 stars
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Shh produced by LPS-stimulated THP-1 cells promotes proliferation of pancreatic cancer cells A, THP-1 cells were cultured in the presence or absence of LPS for 24 h. THP-1 cells were then washed intensively to eliminate LPS and the cell number was readjusted. AsPC-1 or SUIT-2 was cocultured with non-stimulated THP-1 cells, LPS-stimulated THP-1 cells or LPS-stimulated THP-1 cells with 5E1 or IgG antibody for 4 days. Total cell number was counted with a Coulter counter. The fractions of THP-1 cells and PDAC cells were determined using a FACSCaliburTM and the absolute number of PDAC cells was calculated. b AsPC-1 or SUIT-2 was transfected with siRNA for Shh or control siRNA by Lipofectamine for 36 h, and the expression of Shh mRNA was evaluated by real-time PCR (left panels). Shh-silenced AsPC-1 or SUIT-2 was cocultured with non-stimulated THP-1 cells, LPS-stimulated THP-1 cells, or LPS-stimulated THP-1 cells with 5E1 or IgG antibody for 4 days. Total cell number was counted with a Coulter counter. The fractions of THP-1 cells and PDAC cells were determined using a FACSCaliburTM and the absolute number of PDAC cells was calculated. Bars SD.*P < 0.05. c AsPC-1 or SUIT-2 was transfected with siRNA for Smo or control siRNA by Lipofectamine for 36 h, and the expression of Smo mRNA was evaluated by real-time PCR (left panels). Wild-type, control siRNA or Smo-siRNA transfected AsPC-1 or SUIT-2 was cocultured with LPS-stimulated THP-1 cells for 4 days. Total cell number was counted with a Coulter counter. The fractions of THP-1 cells and PDAC cells were determined using a FACSCaliburTM and the absolute number of PDAC cells was calculated. Bars SD. *P < 0.05

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Nuclear factor kappaB-activated monocytes contribute to pancreatic cancer progression through the production of Shh

doi: 10.1007/s00262-009-0783-7

Figure Lengend Snippet: Shh produced by LPS-stimulated THP-1 cells promotes proliferation of pancreatic cancer cells A, THP-1 cells were cultured in the presence or absence of LPS for 24 h. THP-1 cells were then washed intensively to eliminate LPS and the cell number was readjusted. AsPC-1 or SUIT-2 was cocultured with non-stimulated THP-1 cells, LPS-stimulated THP-1 cells or LPS-stimulated THP-1 cells with 5E1 or IgG antibody for 4 days. Total cell number was counted with a Coulter counter. The fractions of THP-1 cells and PDAC cells were determined using a FACSCaliburTM and the absolute number of PDAC cells was calculated. b AsPC-1 or SUIT-2 was transfected with siRNA for Shh or control siRNA by Lipofectamine for 36 h, and the expression of Shh mRNA was evaluated by real-time PCR (left panels). Shh-silenced AsPC-1 or SUIT-2 was cocultured with non-stimulated THP-1 cells, LPS-stimulated THP-1 cells, or LPS-stimulated THP-1 cells with 5E1 or IgG antibody for 4 days. Total cell number was counted with a Coulter counter. The fractions of THP-1 cells and PDAC cells were determined using a FACSCaliburTM and the absolute number of PDAC cells was calculated. Bars SD.*P < 0.05. c AsPC-1 or SUIT-2 was transfected with siRNA for Smo or control siRNA by Lipofectamine for 36 h, and the expression of Smo mRNA was evaluated by real-time PCR (left panels). Wild-type, control siRNA or Smo-siRNA transfected AsPC-1 or SUIT-2 was cocultured with LPS-stimulated THP-1 cells for 4 days. Total cell number was counted with a Coulter counter. The fractions of THP-1 cells and PDAC cells were determined using a FACSCaliburTM and the absolute number of PDAC cells was calculated. Bars SD. *P < 0.05

Article Snippet: The pCMV-IκBα wild type (WT) and pCMV-IκBα mutant were purchased from BD Biosciences/Clontech (Palo Alto, CA).

Techniques: Produced, Cell Culture, Transfection, Expressing, Real-time Polymerase Chain Reaction